Part:BBa_K2483002

Lac regulated dCas9 with LacI constitutively expressed

This part is designed to produce dCas9 when Lac or IPTG is present. LacI-LVA (BBa_C0012: https://parts.igem.org/Part:BBa_C0012) is constitutively expressed under the pRep4 promoter and dCas9 has the Lac promoter (BBa_R0010: https://parts.igem.org/Part:BBa_R0010) at the 5'-end.

This part was used in our metabolic channelling project and is also part of BBa_K2483004: https://parts.igem.org/Part:BBa_K2483004, our final construct for our experiments.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 2666
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1258
  Illegal BamHI site found at 4945
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]